Make it visible

Soft tissue within small insect heads are hard to investigate. Muscles, glands and the brain are accessible by opening the head or cutting the head into thin slices.

I produced a chemical procedure that makes the surface of small insect heads durable for confocal laser-scanning microscopy (CLSM). Thus, I was able to scan whole insect heads and reconstruct soft tissue, like glands and individual neuropiles.

I also used fluorescent dyes for backfills, which appeared to work with this procedure.